Table 2 Amino acid changes during serial passaging

S

27

S

S

S

L

L

S

119

A

A

A

A

V

158

M

M

I

M

M

156

A

A

A

V

A

467

P

P

P

H

S

286

A

A

A

A

S

487

I

Y

I

I

I

300

N

N

N

K

N

488

S

L

S

S

S

306

V

V

V

I

V

537

N

N

S

N

N

426

F

F

L

F

F

574

F

F

F

L

F

433

D

N

D

D

D

636

E

E

K

K

K

448

D

D

A

A

A

652

Y

Y

Y

Y

C

476

V

V

V

A

V

665

L

L

L

P

L

519

A

A

A

V

A

887

S

S

S

S

R

689

V

V

V

V

A

888

G

G

R

R

R

764

Y

Y

F

F

F

969

A

A

S

S

S

830

C

C

C

R

C

1156

T

T

T

A

T

887

S

S

R

R

R

1243

D

E

D

D

D

969

A

A

S

S

S

ORF3

–

–

–

–

–

–

981

L

L

L

L

P

E

51

I

T

I

I

I

1037

K

K

E

E

K

62

S

F

S

S

S

1260

N

N

D

D

D

M

12

I

I

I

I

V

1263

G

C

G

G

G

28

I

T

T

T

T

ORF3

76

F

S

S

S

S

54

I

V

I

I

I

144

I

I

I

I

V

163

S

S

S

S

T

158

N

N

S

N

N

N

106

E

E

E

G

E

188

E

E

D

D

D

179

G

G

G

E

G

E

12

V

V

V

A

V

205

K

K

K

K

R

50

Y

Y

Y

C

C

261

P

P

P

P

S

54

G

G

G

G

R

287

G

G

A

G

G

M

95

Y

Y

Y

H

H

288

E

E

G

E

E

N

157

N

H

H

H

H

424

D

D

D

D

E

216

M

M

M

V

M

223

V

M

M

M

M

286

K

K

K

K

R