Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula

Table 2 Prevalence of Blastocystis subtypes in Spanish wild boars (n = 360) according to the bioregion of origin, type of sampling site, and sampling year.

Variable	Samples (n)	Blastocystis-positive (n)^a	Blastocystis-positive (%)	95% CI (%)	P value	Subtypes detected^b (n)
Bioregion					< 0.001
BR1	21	8	38.1	18.1–61.6		ST5 (8)
BR2	39	9	23.1	11.1–39.3		ST5 (7), ST5/ST15 (1)
BR3	150	13	8.7	4.7–14.4		ST5 (2)
BR4	8	0	0.0	–		–
BR5	142	6	4.2	1.6–9.0		ST5 (4)
Type of sampling site					< 0.001
Hunting state	197	22	11.2	7.1–16.4		ST5 (9), ST5/ST15 (1)
Game reserve	21	8	38.1	18.1–61.6		ST5 (8)
Natural protected area	97	4	4.1	1.1–10.2		ST5 (4)
Urban/suburban	45	2	4.4	0.5–15.2		Not available
Sampling year^c					< 0.001
2014	26	9	34.6	17.2–55.7		ST5 (7), ST5/ST15 (1)
2018	148	6	4.1	1.5–8.6		ST5 (4)
2019	52	6	11.5	4.3–23.4		ST5 (2)
2020	70	5	7.1	2.4–15.9		Not available
2021	60	9	15.0	7.1–26.6		ST5 (7)

95% confidence intervals (95% CI) are included. The values in bold represent statistical significance and subtypes previously reported in humans (regardeless of their true zoonotic potential).
^aSamples were considered positive when Blastocystis was identified after Sanger and next-generation sequencing.
^bSubtype information is only included for the 22 samples in which next-generation amplicon sequencing was conducted.
^cFour samples from unknown sampling years, with one of the samples positive for Blastocystis ST5.

ISSN: 1297-9716