Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula

Table 3 Prevalence of Blastocystis in Portuguese wild boars (n = 99) according to the bioregion of origin and sampling year.

Variable	Samples (n)	Blastocystis positive (n)^a	Blastocystis positive (%)	95% CI (%)	P value	Subtypes detected^b (n)
Bioregion					0.515
BR1	12	3	25.0	5.5–57.2		ST5 (3)
BR2	39	9	23.1	11.1–39.3		ST5 (6), ST5/ST10a (1), ST5/ST14 (1), ST5/ST43 (1)
BR3	48	22	45.8	31.4–60.8		ST5 (18), ST5/ST13 (1), ST5/ST15 (1), ST5/ST24b (1), ST5/ST43 (1)
Sampling year					0.373
2019	64	18	28.1	17.6–40.8		ST5 (14), ST5/ST10a (1), ST5/ST14 (1), ST5/ST24b (1), ST5/ST43 (2)
2020	21	8	38.1	18.1–61.6		ST5 (8)
2021	14	8	57.1	28.9–82.3		ST5 (6), ST5/ST13 (1), ST5/ST15 (1)

All the samples were collected from naturally classified areas. 95% confidence intervals (95% CI) are included. BR1 encompasses Central Portugal West (CPW), BR2 Montesinho Natural Park (MNP), and BR3 Central Portugal East (CPE) and Malcata Nature Reserve (MNR). Subtypes previously reported in humans (regardless of their true zoonotic potential) are in bold.
^aSamples were considered positive when Blastocystis was identified after Sanger and next-generation sequencing.
^bSubtype information obtained from all positive samples via next-generation amplicon sequencing.

ISSN: 1297-9716