Table 1 Detection methods targeting the B646L gene of ASFV
Method | Classifications | Strain | Ref. |
|---|---|---|---|
Based on PCR | A duplex fluorescent quantitative PCR assay | Genotype I and II strains | [132] |
A fast quantitative real-time PCR | ASFV | [46] | |
A novel quantitative real‐time PCR | ASFV-China | [45] | |
Combination of Fe(OH)3 modified diatomaceous earth and qPCR | ASFV in water | [47] | |
Nanofluidic chip digital PCR | ASFV | [105] | |
Based on isothermal amplification | Recombinase-based isothermal amplification assay (RPA/RAA) | Different genotypes ASFV | [70] |
Exonuclease III-assisted recombinase-aided amplification colorimetric assay | ASFV | [72] | |
Semiquantitative colorimetric LAMP method | ASFV and HSV | [52] | |
A LAMP PCR method | ASFV and gene deleted ASFV | [59] | |
RAA nucleic acid test strip assay and double RAA gel electrophoresis detection | ASFV and CSFV | [80] | |
Based on CRISPR | A CRISPR/Cas12a-SERS platform | ASFV | [95] |
CRISPR/Cas12a combined with immunochromatographic strips | ASFV | [133] | |
Cas12a-based portable paper diagnostics | ASFV | [89] | |
CRISPR Cas13a-based lateral flow strip | ASFV | [87] | |
DNA endonuclease-targeted CRISPR transreporter assay | ASFV | [81] | |
CRISPR-Cas12a and fluorescence-based point-of-care system | ASFV | [99] | |
Harnessing multiplex crRNAs in the CRISPR/Cas12a system | ASFV | [101] | |
CRISPR-Cas12a | ASFV | [93] | |
Short palindromic repeats/Cas9-mediated lateral flow nucleic acid assay | ASFV | [96] | |
Cas12a-based on-site detection | ASFV | [82] | |
CRISPR-Cas12a combined with G-quadruplex | ASFV | [98] | |
Combination of CRISPR and isothermal amplification | LAMP assay coupled with CRISPR/Cas12a system | ASFV | [90] |
Visual isothermal amplification on trimeric G-quadruplex cis-cleavage activity of Cas12a | ASFV | [97] | |
CRISPR-Cas12a combined with LAMP or RPA | ASFV | [91] | |
CRISPR/Cas12a-mediated isothermal amplification | ASFV | [65] | |
RPA and Cas12a and Cas13a assay | ASFV | [100] |