Table 2 Detection methods targeting genes of ASFV other than B646L

p54

A novel real-time PCR assay

ASFV

[134]

E248R

A quantitative PCR method

ASFV

[135]

B646L and B438L

A probe-based duplex real-time PCR assay

ASFV

[136]

p72, CD2v and MGF

CRISPR-Cas12a

ASFV

[108]

A137R

Real-time PCR

ASFV

[137]

MGF-360-12L, I177L

Real-time PCR Assays

Highly virulent Georgia strain of ASFV

[138]

E296R

A duplex real-time PCR assay

Genotypes I and II ASFV

[139]

I73R and I329L

The tandem repeat sequences

p72 genotype II and intergenic region

(IGR) II variant

[140]

I73R, I329L, O174L, K145R, I329L, I215L

Sequencing and PCR

24 different European II-ASFV genotypes

[111]

B646L, F1055L, and E183L

A triplex real-time quantitative PCR

Genotypes I and II ASFV

[48]

B646L and E183L

A dual real-time PCR Assay

Genotypes I and II ASFV

[141]

E296R

A duplex real-time PCR assay

Genotypes I and II ASFV

[139]

B646L and MGF505-2R

A duplex TaqMan real-time PCR assay

Genotype II ASFV and MGF505-2R gene-deleted

[117]

B646L, EGFP, and mCherry

A triplex PCR Method

Gene-deleted ASFV

[142]

MGF505-2R, EP402R and I177L

A multiplex real-time quantitative PCR assay

Gene-deleted ASFV

[143]

B646L, MGF505-2R and I177L

A triplex crystal digital PCR

ASFV and the MGF505-2R and I177L Gene-Deleted ASFV

[144]

B646L, MGF_360-14L and CD2v

A triplex real-time quantitative PCR

Gene-deleted and wild-type ASFV

[145]

B646L and EP402R

Amplification assays

Gene-deleted ASFV and wild-type ASFV

[70]

B646L, CSFV 5′ UR, PRRSV ORF7 gene

A multiplex qRT-PCR

ASFV, CSFV, PRRSV

[49]

p72 and Cap protein

CRISPR-Cas12a combined with G-quadruplex

ASFV and PCV2

[98]