Figure 10

Effect ofvtaA31deletion on autoagglutination, biofilm formation and adherence of Δwza. A Expression of VtaA31 in G. parasuis was identified by western blot. M: Protein marker and the red arrow indicate the 156.3 kDa VtaA31 protein. B Liquid culture of the late-logarithmic phase in 50 mL tubes remained stationary for 10 h at room temperature. Samples were taken from the surface of the liquid culture at one-hour intervals, and the OD₆₀₀ value of the samples was measured with a microplate reader. C Each bacteria suspension was added to eight duplicate microtiter plate wells and placed in a 37 ℃ incubator for 24 h. Biofilm formation was determined by staining with crystal violet and measuring the OD590 value after dissolving in ethanol. D Each bacteria suspension was added to four duplicate wells of a 24-well plate. The bacteria adherence to PK15 cells was collected and spread on TSA plates for CFU counting after proper dilution. The adhesion level was expressed as log₁₀ of the average number of CFUs recovered from four wells. The above experiments were performed three times, and the means ± standard deviations from three experiments are shown. Statistical analysis was performed between Δwza or Δwza-vtaA using one-way or two-way ANOVA (asterisks express statistical significance between Δwza and Δwza-vtaA: **** p ≤ 0.0001, ns: non-significant).