Figure 9
Validation of DEGs by qRT-PCR. Fold change of gene expression in Δwza or C-Δwza to ZJ1208 was analysed using the delta-delta comparative threshold cycle (2–ΔΔCt) method, and the relative quantification of gene expression was presented as log2 (2 − ΔΔCt). Δwza(T) and Δwza(Q) represent the results of transcriptome and qRT-PCR analysis, respectively. Statistical analysis was performed between Δwza(Q) or C-Δwza using two-way ANOVA. Data represent the mean ± SD of three independent experiments (asterisks express statistical significance between Δwza and C-Δwza: **** p ≤ 0.0001).