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Figure 1 | Veterinary Research

Figure 1

From: A chimeric strain of porcine reproductive and respiratory syndrome virus 2 derived from HP-PRRSV and NADC30-like PRRSV confers cross-protection against both strains

Figure 1

Design, construction and rescue of the PRRSV MLV strain rGD infectious clone. A Strategy used for constructing the full-length PRRSV MLV strain. B Amplification of four fragments of the whole genome of the PRRSV MLV strain GD by PCR. C Cytopathic effect (CPE) induced by rescued PRRSV rGD in MARC-145 cells. MARC-145 cells were infected with supernatants removed from BHK-21 cells transfected with recombinant pBAC-GD plasmids. Wild-type PRRSV GD was used as a positive control. D The presence of rescued PRRSV rGD was identified by western blotting in MARC-145 cells. E Immunofluorescence assay (IFA) was used to detect the rescued PRRSV rGD strain in MARC-145 cells. Wild-type PRRSV GD was used as a positive control, and DMEM was used as a negative control.

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Veterinary Research

ISSN: 1297-9716