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Figure 3 | Veterinary Research

Figure 3

From: A chimeric strain of porcine reproductive and respiratory syndrome virus 2 derived from HP-PRRSV and NADC30-like PRRSV confers cross-protection against both strains

Figure 3

Chimeric PRRSV rGD-SX-5U2 maintains cell tropism to MARC-145 cells. A CPE induced by rescued chimeric PRRSV rGD-SX-5U2 in MARC-145 cells. MARC-145 cells infected with the rescued chimeric PRRSV rGD-SX-5U2, PRRSV rGD (MARC-145 cells infected) or NADC30-like PRRSV SX-YL1806 (MARC-145 cells not infected) were used as controls. B The infectivity of the rescued chimeric PRRSV strain rGD-SX-5U2 against MARC-145 cells was identified by western blotting. MARC-145 cells were infected with rGD-SX-5U2 (MOI = 0.1) and harvested at 36 dpv. C IFA was used to verify the infectivity of rGD-SX-5U2 in MARC-145 cells. D The virus titre of the rescued chimeric PRRSV rGD-SX-5U2 was determined in MARC-145 cells. MARC-145 cells were infected with rGD-SX-5U2 or rGD (MOI = 0.1). E Representative images of the plaque morphologies of rGD-SX-5U2 in MARC-145 cells; PRRSV rGD and SX-YL1806 were used as controls.

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Veterinary Research

ISSN: 1297-9716