Table 1 Primers used for construction of the PRRSV MLV strain rGD or chimeric PRRSV rGD-SX-5U2 infectious clones
Primer | Sequence | Product size(bp) |
|---|---|---|
F1-F | AGTGAACCGTGGCCCGGGCGGCCATGACGTATAGGTGTTGGCTCT | 1937 |
F1-R | ACTGCCGGACATTTCCGCCAGGGCTGCCGGGATGACACTACTAGGC | |
F2-F | TCCCGGCAGCCCTGGCGGAAATGTCCGGCAGTTTTGGTTGTTCAACACCT | 3954 |
F2-R | CTTCCAGTTCGGGTTTGGCAGCAAGCAGGGCACAA | |
F3-F | AACCCGAACTGGAAGGAGGCCTTTCCACAGTTCAACT | 5400 |
F3-R | CGGCCTTCAAGTTGAAAATAGGCCGTCTTGTCTTTCCATAC | |
F4-F | ATTTTCAACTTGAAGGCCGCCATTTTACCTGGTATCAACTTGCAAG | 3889 |
F4-R | CGGATGCCCAGGTCGGACCGCGAGGAGGTGGAGATGCCATGCCGACCCTTTTTTTTTTTTTTTTTTTTTTTTTAATTTCGGCCGCATGGTTCTCGCCAATT | |
Fragement A-F | GGCAATTTGAATGTTCAAGTATGTTGGGGAAATGCTTGACCG | 1516 |
Fragement A-R | GATGTCTCAAGAATGTCAGCCCATCATGCTGAAGGTGGCGTTGTG | |
Fragement B-F | CTGACTAAGGAGCAGTGTTTAAACTGCTAGCCGCCAGCGG | 6113 |
Fragement B-R | TGAACATTCAAATTGCCAGTAGGATGGCAAAAAGACAGGCTAAA | |
Fragement C-F | GCTGACATTCTTGAGACATCCTGGTGTTTGAATTGGAAGAATGC | 218 |
Fragement C-R | ATGCCCAGGTCGGACCGCGAGGAGGTGGAGAT | |
Fragement E-F | CATAAGTGGTACGGTGCTGGGAAGAGAGCAAGAAGAGCA | 3222 |
Fragement E-R | GAGGTGTGGGCCTCCTCCTGAAGACTTGGAGATCTGCCT | |
Fragement F-F | ACCGTGGCCCGGGCGGCCATGACGTATAGGTGTTGGCTCT | 1361 |
Fragement F-R | CCAGCACCGTACCACTTATGACTGCCAAACCG | |
Fragement G-F | TTCAGGAGGAGGCCCACACCTCATTGCTGCC | 2779 |
Fragement G-R | GTTTAAACACTGCTCCTTAGTCAGGCCTTGGAGTTTGTC |