Table 2 List of primers used for PCR and DNA sequencing
Primers | Sequence (5′–3′) | Tage gene | |
|---|---|---|---|
spc-F | TTCGTATGCCGTCTTCTG | SpcR | |
spc-R | CTGGCAGTTCCCTACTCT | ||
capD-Up-F | ACTGCATGAATTCCCGGGAGTCAAGAACGATGAGGTGT | Upstream fragment of capD | |
capD-Up-R | CAGAAGACGGCATACGAAAACGCTCGCTGTAAATAG | ||
capD-Down-F | AGAGTAGGGAACTGCCAGAAAATGATAGTGCCAGAG | Downstream fragment of capD | |
capD-Down-R | CGATCCCAAGCTTCTTCTAGGCAATAACTAATGCCAAG | ||
neutral site-Up-F | TACTCAGAGCTCGGTGGAGCACCTATGT | Upstream fragment of neutral site | |
neutral site-Up-R | TACTCAGGATCCTCGTATGAGGGCTCTT | ||
neutral site-Down-F | TACTCAGTCGACGGGCTCTGTAATAATTCGGG | Downstream fragment of neutral site | |
neutral site-Down-R | TACTCAGGTACCGGAACCCCTGCTCAAA | ||
PompA-F | AGCCCTCATACGAGGATCCCTTGCCACATTTGGTG | ompA gene promoter | |
PompA-R | CAGTTCTTCGCCTTTGCTC | ||
capD-F2 | GAGCAAAGGCGAAGAACTGCTGCCAAGGTGGATTGTTCT | Full-length sequence of capD | |
capD-R2 | TTATTACAGAGCCCGTCGACGGAGTGCTGTTTCGGTAGCT | ||
capD-F | AATCCTAGCCTGCCTTAT | Internal sequence of capD | |
capD-R | AGTTGACGCACTACTTCT | ||
Check-knockout-F | ACGTAGGATTTACTCTGG | External sequence of capD | |
Check-knockout-R | AACAACTGACCTGATGAG | ||
Check-knockin-F | GATAGAACTTGCAGACGG | External sequence of neutral site | |
Check-knockin-F | TTCAGAGTAGTGCACAGG | ||