Figure 2
Recombinant virus rescue and extracellular characterisation of the gI N-glycosylation site mutation in the DPV CHv strain. A Schematic representation of the gI N-glycosylation site mutation in the DPV CHv strain. B Following the transfection of plasmids containing the gI mutational infectious clones (pDPV CHv-gIN69A, pDPV CHv-gIN78A, pDPV CHv-gIN265A, pDPV CHv-gIN69/78/265A, pDPV CHv-gIN69/78/265A repair, and pDPV CHv△gE-gIN69/78/265A), the successful rescue of the virus was indicated by the observation of EGFP under a fluorescence microscope, while CPE was monitored using a light microscope. Cells transfected with the mock plasmid served as the control group. C Crystal violet staining was utilised to assess viral plaques. D Fifteen plaques were randomly selected per group for measurement of plaque diameter using Image J software (ns indicates not significant, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; t-test).