Figure 4
Subcellular localisation of recombinant gI protein. (1) The recombinant gI plasmids (pCAGGS-gI-Flag, pCAGGS-gIN69A-Flag, pCAGGS-gIN78A-Flag, pCAGGS-gIN265A-Flag, and pCAGGS-gIN69/78/265A-Flag) were co-transfected with pDsRed2-ER into DEFs. (2) The pCAGGS-gI-Flag and pCAGGS-gIN69/78/265A-Flag plasmids were individually transfected into DEFs, while the pCAGGS-gIN69/78/265A-Flag and pCAGGS-gE-MYC plasmids were co-transfected into DEFs, and their localisation was marked using GM130. (3) DPV CHvΔgE-gIN69/78/265A and DPV CHv-gIN69/78/265A were respectively used to infect DEFs, and their localisation was similarly marked using GM130. Transfection or infection was carried out, and IFA was performed after 36 h. Fluorescence microscopy was used for visualisation (magnification: 600 × ; scale bar: 20 μm).