Figure 6

In vivo experiments. A Schematic representation of the experimental procedure investigating the impact of gI N-glycosylation on DPV. At 14 days old, ducks were intramuscularly injected with DPV CHv₅₀, DPV CHv-gI_N69/78/265A, DPV CHv-gI_N69/78/265A repair, and MEM (10⁶ TCID₅₀). Ducks injected with MEM served as negative controls. Group 1 (n = 40, 10 ducks per group) was used to determine the survival rate, rectal temperature, and body weight of the ducks. Group 2 (n = 40, 10 ducks per group) was euthanised at 2, 5, 8, and 10 days post-inoculation to assess pathological changes in different organs and viral load. B–D Survival curves (B), rectal temperature (C), and body weight (D) of ducks inoculated with the specified viruses. The data were analysed using the Mantel-Cox log-rank test.