Table 1 Primer sequences for overlapping PCR and RT-qPCR
Name | Purpose | Sequence (5′–3′) |
|---|---|---|
pCAGGS-gI-Flag-F | Construct pCAGGS-gI-Flag plasmid | 5ʹ- CATCATTTTGGCAAAGAATTCGCCACCATGGGAACGACACGACATATACTGATA-3ʹ |
pCAGGS-gI-Flag-R | 5ʹ-TTGGCAGAGGGAAAAAGATCTTTACTTGTCATCGTCGTCCTTGTAGTCCTTGTCATCGTCGTCCTTGTAGTCTTCTGTTTTATGATCCCCAGA-3ʹ | |
pCAGGS-gIN69A-Flag-F | overlapping PCR | 5ʹ-CTACCGCCAGTATACGCGGGAACTGTCGAGCTA-3ʹ |
pCAGGS-gIN69A-Flag-R | 5ʹ-CGCGTATACTGGCGGTAGTTCGGCCTGTGATCC-3ʹ | |
pCAGGS-gIN78A-Flag-F | overlapping PCR | 5ʹ-GAGCTACTAGTATATGCGATTTCTCGTCACTGC-3ʹ |
pCAGGS-gIN78A-Flag-R | 5ʹ-CGCATATACTAGTAGCTCGACAGTTCCATTGTA-3ʹ/ 5ʹ-CGCATATACTAGTAGCTCGACAGTTCCCGCGTA-3ʹ | |
pCAGGS-gIN265A-Flag-F | overlapping PCR | 5ʹ-ATGCAGCCTACGCATGCGGATACCAATAGTGGA-3ʹ |
pCAGGS-gIN265A-Flag-R | 5ʹ-CGCATGCGTAGGCTGCATATCATCAGATAATGT-3ʹ | |
pCAGGS-gIN69/78/265A-Kan-F | The Kan fragment was introduced into the recombinant plasmid | 5ʹ-GACGACGATGACAAGTAAAGATCTTTCACATCAGTATGACTAAAAGTAAATGTTTTTGTTATGATTGACTGTTTTAGGGATAACAGGGTAATCGATTT-3ʹ |
pCAGGS-gIN69/78/265A-Kan-R | 5‘-TTGGCAGAGGGAAAAAGATCTGCCAGTGTTACAACCAAT-3ʹ | |
gI-repair-F | Amplified complement fragments | 5ʹ-CGTACTTCCAGTATTGTCCAGTGCGCCATATAGACGATATATTGAGTTTCAAAAATAGAAATGGGAACGACACGACATATA-3ʹ |
gI-Kan- repair-R | 5ʹ-TACAAATATATTTGGATGTTAATGAAAGGCAAACAGTCAATCATAACAAAAACATTTACTTTTAGTCATACTGATGTGAAGCCAGTGTTACAACCAAT-3ʹ | |
UL30-F | RT-qPCR | 5ʹ-TTTTCCTCCTCCTCGCTGAGT-3ʹ |
UL30-R | 5ʹ-GGCCGGGTTTGCAGAAGT-3ʹ |