Figure 2

Changes in the antibacterial activity of cc-CATH3, ML-CATH, and PD-CATH after incubation with naïve serum. cc-CATH3, ML-CATH, and PD-CATH peptides were incubated in 50% (v/v) FBS each for 0, 60, and 120 min before applying to antimicrobial activity assay against E. coli (ATCC 25922). The antimicrobial activity was determined by measuring bacterial growth at OD 450 nm. The X and Y axes indicate peptide concentration and absorbance at 450 nm, respectively. Samples containing 50% FBS in the absence of the peptides indicate a control for bacterial growth without AMP treatments. The antimicrobial activity of cc-CATH3 (A), ML-CATH (B), and PD-CATH (C) after the treatment of FBS-incubated AMPs was shown.