Bactericidal activities and biochemical features of 16 antimicrobial peptides against bovine-mastitis causative pathogens

Table 1 The physicochemical characteristics of analysed cathelicidins in this study

Names	Origin	Sequences	Length	Molecular weight (Da)	Net charge at pH 7	Hydrophobic residue (%)	Cationic residue (%)	GRAVY score
ΔModoCath1	Monodelphis domestica	VKRTKRGARRGLTKVLKKIFGSIVKKAVSKGV	32	3510.37	11.9	37.50	37.50	− 0.31
ΔModoCath5		WYQLIRTFGNLIHQKYRKLLEAYRKLRD	28	3623.27	5.1	35.71	28.57	− 0.85
ΔModoCath6		VRRSKRGIKVPSFVKKVLKDVVSESIS	27	3042.66	5.9	40.74	29.63	− 0.19
PMAP-36	Sus scrofa	GRFRRLRKKTRKRLKKIGKVLKWIPPIVGSIPLGCG	36	4157.22	12.9	41.67	36.11	− 0.46
PG-1^a		MRGGRLCYCRRRFCVCVGR	19	2291.82	5.8	22.22	33.33	− 0.25
PR-35		RRRPRPPYLPRPRPPPFFPPRLPPRIPPGFPPRFP	35	4223.1	8.9	68.57	25.71	− 1.32
ΔPb-CATH4	Python bivittatus	TRSRWRRFIRGAGRFARRYGWRIA	24	3052.55	8.9	37.50	37.50	− 1.10
ΔHg-CATH	Heterocephalus glaber	SKFFRKARKKLGKGLQKIKNVLRKY	25	3035.77	10.9	32.00	44.00	− 1.08
BMAP-28	Bos taurus	GGLRSLGRKILRAWKKYGPIIVPIIRIG	28	3131.89	6.9	50.00	25.00	0.23
EA-CATH1	Equus asinus	KRRGSVTTRYQFLMIHLLRPKKLFA	25	3060.75	7.1	44.00	32.00	− 0.35
cc-CATH3	Coturnix coturnix	RVRRFWPLVPVAINTVAAGINLYKAIRRK	29	3379.11	6.9	58.62	24.14	0.14
HA-CATH	Hipposideros armiger	ILGRLRDLLRRGGRKIGQGLERIGQRIQGFFSNREPMEES	40	4640.37	3.9	32.50	22.50	− 0.82
ML-CATH	Myotis lucifugus	LNPLIKAGIFILKHRRPIGRGIEITGRGIKKFFSK	35	3975.87	8.1	45.71	28.57	− 0.04
PD-CATH	Phyllostomus discolour	ILGPALRIGGRIAGRIAGKLIGDAINRHRERNRQRRG	37	4088.79	8.2	37.84	29.73	− 0.65

^aThe peptide has an additional methionine at N-terminus as it was produced by a recombinant expression system [22].

ISSN: 1297-9716