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Figure 2 | Veterinary Research

Figure 2

From: A pH-triggered self-releasing humic acid hydrogel loaded with porcine interferon α/γ achieves anti-pseudorabies virus effects by oral administration

Figure 2

Expression, purification, toxicity assessment, and antiviral activity evaluation of the rPoIFNα/γ recombinant protein. A SDS‒PAGE analysis of the purified rPoIFNα/γ protein. B Western blot analysis of the rPoIFNα/γ protein. C Cytotoxicity of various concentrations of rPoIFNα/γ on PK-15 cells was assessed after 24 h of incubation. Statistical significance was determined using Student’s t tests (***p < 0.001; ns, not significant). D Antiviral activity of rPoIFNα/γ against vesicular stomatitis virus (VSV) and pseudorabies virus (PRV) was assessed in vitro using the cytopathic effect inhibition method.

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Veterinary Research

ISSN: 1297-9716