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Figure 8 | Veterinary Research

Figure 8

From: A pH-triggered self-releasing humic acid hydrogel loaded with porcine interferon α/γ achieves anti-pseudorabies virus effects by oral administration

Figure 8

IFNα/γ@PAMgelinduced ISG (Pkr, Ifit1, and Isg20) expression in mice. The spleens were collected at the indicated time points. RT‒qPCR analysis of Pkr (A), Ifit1 (B), and Isg20 (C) mRNA in mice from the fresh spleen samples of the IFNα/γ@PAMgel and control groups collected on days 1, 3, 5, and 7. Mice in the treatment group were administered IFNα/γ@PAMgel orally for three days, and the controls were untreated. Total RNA from the spleen tissue was extracted using RNA Plus reagent and reverse transcribed into cDNA. Relative gene expression analysis was performed using the 2.−ΔΔCt method, and β-actin was used as an endogenous control. The data are representative of three independent experiments analysed by Student’s t tests (** p < 0.01; *** p < 0.001; ns, not significant).

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Veterinary Research

ISSN: 1297-9716