Table 1 Primers and siRNA in this study
Primers and siRNA | Sequence (5’-3’) |
|---|---|
siHsp70-1 | GGCCUUUCCAGGUGAUCAATT |
siHsp70-2 | GCGCAACGUGCUCAUCUUUTT |
siHsp70-3 | CCAAGCAGACGCAGAUCUUTT |
siHsp70-4 | UGCACCUUGGGCUUGUCUCCGUCGU |
siHsp70-5 | AGGCCAACAAGAUCACCAUTT |
siHsp70-6 | GGUGCUGACCAAGAUGAAGTT |
siCon | UUCUCCGAACGUGUCACGUTT |
Flag-Hsp70-F | TTGTCGACTATGGCCAAAGCCGCGGCGAT |
Flag-Hsp70-R | TTGGTACCCTAATCCACCTCCTCAATGGTAGGGC |
Flag-Hsp70 (ΔSBD)-F | CAGGACCTGCTGCTGCTGAGCAAGGAGGAG |
Flag-Hsp70 (ΔSBD)-R | CTCCTCCTTGCTCAGCAGCAGCAGGTCCTG |
Flag-Hsp70 (ΔNBD)-F | ATTCGGTCGACTATGAACGTGCAGGACCTG |
Flag-Hsp70 (ΔNBD)-R | AGGTCCTGCACGTTCATAGTCGACCGAATT |
Myc-L-F | TTGAATTCGGATGCAGAACTCTCATTTTTCTTTCG |
Myc-L-R | TTGGTACCTTACTGCAGCTCGTATACGATGTCCAG |
Myc-3D-F | TTGAATTCCGATGGGACTAATGACTGAGCTAGAGCC |
Myc-3D-R | TTGGTACCTTATCAGTCGAACAAGGCCCTCCATC |
GFP-3D (aa1-150)-F | TTGAATTCAGGACTAATGACTGAGCTAGAGCC |
GFP-3D (aa1-150)-R | TTGGTACCGTCACCGTCTAAGAATCTCTGG |
GFP-3D (aa149-298)-F | TTGAATTCAGGTGACTACTCTGATCATGTCT |
GFP-3D (aa149-298)-R | TTGGTACCGGCACAACCAGAGGGGAGGC |
GFP-3D (aa297-463)-F | TTGAATTCATGTGCCGCGACCAGCCTGCT |
GFP-3D (aa297-463)-R | TTGGTACCTCAGTCGAACAAGGCCCTCCAT |