Veterinary Research

Table 1 Assessment of Cq value cut-off for defining A. pleuropneumoniae qPCR-positive samples as clinical cases

From: Serotype diversity of Actinobacillus pleuropneumoniae detected by real-time PCR in clinical and subclinical samples from Spanish pig farms during 2017–2022

Cq value cut-off	Se	CI 95%		Sp	CI 95%		PPV	NPV
38	98.5	0.95	1.00	78.6	0.75	0.82	58.79	99.4
32	94.9	0.91	0.97	92.5	0.90	0.94	79.57	98.33
30	94.4	0.90	0.97	94	0.92	0.96	83.04	98.2
28	92.4	0.88	0.96	95	0.93	0.96	85.05	97.58
26	89.8	0.84	0.93	96.1	0.94	0.97	87.62	96.84
24	88.8	0.83	0.93	95.9	0.94	0.97	87.06	96.52
22	74.1	0.67	0.80	97.3	0.96	0.98	89.57	92.4
20	43.1	0.36	0.50	98.4	0.97	0.99	89.47	84.84

Se: sensitivity, Sp: specificity, PPV: positive predictive value, NPV: negative predictive value.

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ISSN: 1297-9716

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