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Figure 5 | Veterinary Research

Figure 5

From: DPV pUL15 possesses a potential NLS, which is important for the location of the terminase complex and for viral proliferation and genome cleavage

Figure 5

Mutation of the DPV pUL15 NLS impaired the nuclear translocation of terminase subunits but not the interaction between terminase subunits. A Schematic of DPV pUL15 NLS mutants. The basic amino acids (K or R) were replaced with glutamine (Q). (B) Mutations in pUL15 prevented the nuclear transportation of terminase subunits. pcDNA3.1-UL15K185Q, pcDNA3.1-UL15K187Q, pcDNA3.1-UL15R188Q, pcDNA3.1-UL15K190Q and pcDNA3.1-UL15∆NLS were co-transfected with pCMV-UL28 and pCMV-UL33 into DF-1 cells. Anti-His, anti-HA, and anti-Myc antibodies were used to detect the distribution of the pUL15 mutant, pUL28, and pUL33, respectively. C Statistical analysis of the different intracellular distributions of the terminase subunits shown in Figure 5B. The percentages of cells with only cytoplasmic staining, only nuclear staining, or nuclear and cytoplasmic staining corresponding to Figure 5B were determined by counting 50 fluorescent cells per group. D The interaction between pUL15 mutants, pUL28 and pUL33. The pUL15 mutants pUL28 and pUL33 were co-expressed in HEK293T cells as indicated. The interaction was detected by co-IP and Western blot analyses.

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Veterinary Research

ISSN: 1297-9716