Figure 6

DPV pUL15 K187, R188 and K190 are important for viral proliferation and genome cleavage. AÂ Schematic diagram of recombinant infectious clone construction. BÂ Growth curves of DPV pUL15 mutants. CÂ Schematic of TaqMan dual real-time PCR. The UL31-P-6-FAM probe was used to detect the number of total genome copies, and the CTJ-P-6-VIC probe was used to detect the number of concatemeric genome copies. DÂ Number of concatemeric genome copies of WT, rUL15K187Q, rUL15R188Q, and rUL15K190Q at 18Â h post-infection. EÂ The total number of genome copies of WT, rUL15K187Q, rUL15R188Q, and rUL15K190Q was detected at 18Â h post-infection. FÂ The ratio of concatemeric genome copy number to total number of genome copies.