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Table 2 Primers for infectious clone construction

From: DPV pUL15 possesses a potential NLS, which is important for the location of the terminase complex and for viral proliferation and genome cleavage

Primers

Primer sequence (5′- 3′)

pUL15K187Q-Kan-F/R

F: TTTCCAAGATGCTGGGACGGATGCAAATACTATAAAGGCACAAAGAAGTAAGGTCGATGTGCCTAGGGATAACAGGGTAATCGATTT

R: CGCGCATCTTTCCATAAGTTGGCACATCGACCTTACTTCTTTGTGCCTTTATAGTATTTGCATGCCAGTGTTACAACCAAT

pUL15R188Q-Kan-F/R

F: CCAAGATGCTGGGACGGATGCAAATACTATAAAGGCAAAGCAAAGTAAGGTCGATGTGCCAACTAGGGATAACAGGGTAATCGATTT

R: TACCGCGCATCTTTCCATAAGTTGGCACATCGACCTTACTTTGCTTTGCCTTTATAGTATTTGGCCAGTGTTACAACCAAT

pUL15K190Q-Kan-F/R

F: TGCTGGGACGGATGCAAATACTATAAAGGCAAAGAGAAGTCAAGTCGATGTGCCAACTTATGGTAGGGATAACAGGGTAATCGATTT

R: CAAGAGTACCGCGCATCTTTCCATAAGTTGGCACATCGACTTGACTTCTCTTTGCCTTTATAGGCCAGTGTTACAACCAAT

UL15NLS-F/R

GAGGAAGAAACGATAGCACAT

ATGTCTCAGCGATGTTTCAGA

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Veterinary Research

ISSN: 1297-9716