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Figure 1 | Veterinary Research

Figure 1

From: Impact of sodium alginate hydrogel containing bacteriophage peptides that specifically bind to the EtCab protein on the inhibition of Eimeria tenella infection

Figure 1

Detection of prepared polyclonal antisera against EtCab protein. The titre of the prepared antisera against the EtCab protein was 1:216. The positive cutoff = 2 × OD450 value of negative control (A). Western blot analysis was performed to detect the specific reaction of the anti-EtCab antisera with E. tenella sporozolites, revealing a band of 66 kDa (B). An indirect immunofluorescence assay (IFA) showed that the anti-EtCab antisera specifically reacted with the EtCab protein of E. tenella sporozoites, producing a green fluorescence signal (C). The titre of each sample was tested in triplicate, and the values are presented as mean ± SD (n = 5).

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Veterinary Research

ISSN: 1297-9716