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Figure 4 | Veterinary Research

Figure 4

From: Impact of sodium alginate hydrogel containing bacteriophage peptides that specifically bind to the EtCab protein on the inhibition of Eimeria tenella infection

Figure 4

Inhibition of sporozoites invasion into MDBK cells by target peptides in vitro. The maximum non-toxic concentration of the three peptides was found to be 125 µg/mL (A). The labelled sporozoites were incubated with anti-EtCab antisera diluted 1:100, 1:200, and 1:300, respectively, before being added to MDBK cells. The dilution of anti-EtCab antisera at 1:100 resulted in the lowest invasion rate and the highest rate of invasion inhibition (B). Additionally, the labelled sporozoites were incubated with concentrations of 125, 75, and 25 μg/mL of the three peptides, and then introduced to MDBK cells. At a concentration of 125 μg/mL, peptides Y and G exhibited a higher rate of invasion inhibition (C). Each sample was tested in triplicate, and the values are expressed as mean ± SD (n = 3). Statistical significance is indicated as follows: *P < 0.05, **P < 0.01, ***P < 0.001, and “ns” indicates no statistical difference.

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Veterinary Research

ISSN: 1297-9716