Figure 9

Prediction of 3D model for EtCab protein and molecular docking with target peptides. Molecular docking analysis was conducted to evaluate the binding of Y (B), G (C), and V (D) peptides to the EtCab protein. The EtCab protein is shown in green, while the polypeptides are highlighted in red. Dotted lines indicate hydrogen bonds, and the numbers reflect bond lengths (with shorter bond lengths correlating to greater bond energy). The label ASN-3 specifies that position 3 contains an ASN amino acid. The I-Tasser server generated the top four models and their corresponding ratings (A). The sequence alignment of Cab protein amino acids from five Eimeria species is displayed. Amino acids with a red shadow are completely conserved, those in red font red are partially conserved, amino acids bound to the short peptide are highlighted in green, and amino acids that interact with the Y and G peptides are indicated in blue (E).