Impact of sodium alginate hydrogel containing bacteriophage peptides that specifically bind to the EtCab protein on the inhibition of Eimeria tenella infection

Table 4 Amino acids that predicted for forming hydrogen-bonds between EtCab protein and peptide Y, G and V

Peptides corresponding to affinity phages	Number of hydrogen-bonds	Amino acids contribute for forming hydrogen-bonds in target peptides	Common amino acids in EtCab protein contribute for forming hydrogen-bonds with
Peptides corresponding to affinity phages	Number of hydrogen-bonds		Lys¹⁷⁴ (underlined)	Asp¹⁷⁵ (shadow)
Y	13	Try¹-Ala⁸², Try¹-His³⁰¹, *Asn³-Asp¹⁷⁵(2), Ile⁵-Lys¹⁷⁴, Asn⁶-Asp¹⁷³, Asn⁶-Ala¹⁷⁰, Arg⁸-Glu¹⁶⁷(3), Arg⁸-Glu²⁵⁹, Arg*⁸-Thr²⁶⁷, Thr¹⁰-Ser²⁶⁴	●	◆
G	7	Asp²-Lys¹⁷⁴, *Arg³-Asp¹⁷⁵, Arg³-Asp¹⁸³(2), Arg*³-Glu¹⁸⁴, Ile⁷-Lys¹⁷⁴, Trp⁸-Asp²⁹⁵	●	◆
V	9	Val¹-Glu¹²⁶(3), *Asn³-Glu²⁷, Gly⁴-Glu²⁷, Phe⁵-Val²³, His⁶-Ser³³(2), Arg*¹²-Glu¹²²

Try¹-Ala⁸² means hydrogen-bond was formed between position 1 amino acid Try in Y peptide chain and position 82 amino acid Ala in EtCab protein chain. Asn³-Asp¹⁷⁵(2) means two hydrogen-bonds were formed between the two amino acids.
Common amino acids in three short peptides that can form hydrogen bonds with EtCab proteins are marked with italics and shadow.

ISSN: 1297-9716