Figure 3

Deletion of fliC reduces IL-6 IL-8 secretion in infected epithelial cells. The supernatants of cell cultures were harvested 3 h and 6 h post-infection of Salmonella for the detection of pro-inflammatory cytokines. A–D HeLa cells were infected with ST34 S. Typhimurium and Salmonella 4,[5],12:i:- strains. E–H HeLa cells were infected with ST19 S. Typhimurium and Salmonella 4,[5],12:i:- strains. The N/I group represents the uninfected group. The experiment was performed three times in duplicates. Error bars represent ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.