Figure 5
The release of LDH induced by fliC mutants presents opposed changes in HeLa and RAW264.7. A, C Cells were infected with ST34 S. Typhimurium and Salmonella 4,[5],12:i:- strains. B, D Cells were infected with ST19 S. Typhimurium and Salmonella 4,[5],12:i:- strains. The N/I group represents the uninfected group. Cell cytotoxicity was quantified using the LDH Cytotoxicity Assay Kit. The experiment was performed three times in duplicates. Error bars represent ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.