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Figure 1 | Veterinary Research

Figure 1

From: Expression of recombination antimicrobial protein PIL22-PBD-2 in Pichia pastoris and verification of its biological function in vitro

Figure 1

Construction and expression of recombinant PIL22-PBD-2 protein in P. pastoris. A Schematic diagram of recombinant proteins design. B Identification of the plasmids pPIC9k-PIL22-PBD-2 by double enzyme digestion. M: DL10000 DNA marker; Lane 1: plasmid p9k-PIL22-PBD-2 digested with EcoR I-Not I; Lane 2: p9k-PIL22-PBD-2 without EcoR I-Not I. C Colony PCR identification of recombinant P. pastoris containing PIL22-PBD-2 gene. Lane M: DL2000 DNA marker; Lane N: negative control; Lane 1 and 2: P. pastoris GS115; Lane 3 and 4: P. pastoris GS115 containing PIL22-PBD-2 gene; SDS-PAGE (D) and WB (E) analyses of PIL22-PBD-2 protein. Lane M: protein marker. Lane 1: the fermentation supernatant of PIL22-PBD-2. Lane 2: the fermentation supernatant of blank vector as negative control; Lane 3: purified PIL22-PBD-2 protein.

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Veterinary Research

ISSN: 1297-9716