Table 3 Primers for 2−ΔΔCt method qRT-PCR in this study.
Gene name | Primer sequences | Origin of Sequences |
|---|---|---|
ZO-1 | F: CCCCAGGGACTGGAAGAAATCAC R: CCCCTCCACTTCCTCTAGCCAAG | [41] |
E-cadherin | F: TGAGAAATGAGTGTGCTTTTGTGCC R: TGGCCAGACTAAGACATGAACCTC | [41] |
PBD-1 | F: CTCCTCCTTGTATTCCTCCT R: GGTGCCGATCTGTTTCAT | [42] |
PBD-2 | F: GACTGTCTGCCTCCTCTC R: GGTCCCTTCAATCTGTTG | [42] |
IL-6 | F: CCTCGGCAAAATCTCTGCAA R: TGAAACTCCACAAGACCGGT | [43] |
TNF-α | F: GCCCTTCCACCAACGTTTTC R: TCCCAGGTAGATGGGTTCGT | [43] |
β-actin | F: AGATCAAGATCATCGCGCCT R: ATGCAACTAACAGTCCGCCT | [43] |