Figure 2
From: Development of a reporter feline herpesvirus-1 for antiviral screening assays
Stability of the double-reporter FHV-1 in CRFK cells. A Double-reporter viruses (P0, P5, P10, P15, and P20) can infect CRFK cells and express mNeonGreen. The fluorescent protein was observed by fluorescence microscopy at 48 hpi. B Plaque sizes of viruses P0, P5, P10, P15, and P20. C The specific PCR primers for amplifying the Gluc-2A-mNeonGreen of viruses P0, P5, P10, P15, and P20. The identified primer-F was located in the Gluc gene, and the identified primer-R was located in the right arm. D The PCR product of each virus was analysed by agarose gel electrophoresis. E Gluc activity of viruses P0, P5, P10, P15, and P20 infecting CRFK cells at an MOI of 0.005 for 48 h. Statistical analysis, one-way ANOVA (Kruskal‒Wallis test); ns: p > 0.05. F Viral titres of viruses P0, P5, P10, P15, and P20 infecting CRFK cells at an MOI of 0.05 for 48 h. Statistical analysis, one-way ANOVA (Kruskal‒Wallis test), ns: p > 0.05.