Skip to main content
Figure 2 | Veterinary Research

Figure 2

From: African swine fever virus enhances viral replication by increasing intracellular reduced glutathione levels, which suppresses stress granule formation

Figure 2

Intracellular GSH levels were elevated following ASFV infection. (A) Intracellular GSH levels were measured using specific probes, with signal intensity (white light) shown at 3, 12, and 24 hp with an MOI = 5. (B) Thioltracker signal intensity in individual PAMs was quantified and visualised using GraphPad Prism. Each dot represents the values for individual PAM. The longer horizontal lines indicate the mean values, while the error bars represent the standard deviation (SD). (C) PAMs were either mock-infected or ASFV-infected and treated with or without Ars at 12 hpi, with an MOI = 1. The SGs were detected using an anti-G3BP1 antibody, while intracellular GSH levels were labelled with the Thioltracker probe. Red arrows highlight that ASFV-infected PAMs exhibit higher GSH levels than mock-infected or Ars-treated PAMs. (D) GSH intensity was quantified and visualised.

Back to article page

Veterinary Research

ISSN: 1297-9716