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Figure 5 | Veterinary Research

Figure 5

From: African swine fever virus enhances viral replication by increasing intracellular reduced glutathione levels, which suppresses stress granule formation

Figure 5

AIFM1 is an important target for regulating the intracellular antioxidant capacity of ASFV-infected PAMs (MOI = 1). (A) Lysates were harvested from PAMs infected with PBS or ASFV and treated with PBS or Ars, followed by WB at 24 hpi. (B) PAMs were transfected with si-AIFM1 for AIFM1-knockdown and followed by mock- or ASFV-infection. Staining with anti-AIFM1 (green) and anti-p30 (red) was used to visualise the results. (C) SGs formation in AIFM1-knockdown PAMs, with staining using anti-AIFM1 (red) and anti-G3BP1 (green). (D) PAMs were transfected with si-NC or si-AIFM1 for 24 h followed by mock- or ASFV-infection. PAMs were treated with Ars or PBS at 24 hpi followed by a confocal assay using anti-G3BP1 (green) and anti-p30 (red). Red arrows indicate obvious SGs formation in ASFV-infected PAMs under AIFM1-knockdown.

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Veterinary Research

ISSN: 1297-9716