Figure 2

Replication kinetics in blood monocytes and RK-13 cells. Representative confocal microscopy images of blood monocytes and RK-13 cells infected with EHV-1 abortigenic 97P70 and neuropathogenic 21P40 at 1, 12, 24 and 48 hpi (viral proteins in green and CD172a marker in red). A Percentage of infected cells. B Extracellular and intracellular virus titers. Data are presented as means of three experiments. Values with an asterisk are statistically different. At 48 hpi, RK-13 cells were detached. Scale bar 25 μm. Dashed line represents the limit of detection (LOD).