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Figure 5 | Veterinary Research

Figure 5

From: PRV-1b and PRV-3a infection is associated with the same clinical disease in coho salmon (Oncorhynchus kisutch) farmed in Chile: unraveling the pathogenesis of the orthoreoviral cardiomyopathy and hemolytic jaundice (OCHJ)

Figure 5

Immunohistochemistry for PRV-1-infected fish in coho salmon farmed in Chile. A Negative control. Ventricle heart without immunostaining for PRV antigen using σ1 antibody. Bar 50 μm. B Immunostaining in the heart of PRV-1-infected specimens from farm 1 in CP2. PRV antigen detected with σ1 antibody in leukocyte-like cells (red arrow) and in cardiomyocytes of the stratum spongiosum of the ventricle (black arrow). Bar 50 μm. Enlarged inset shows an image of an erythrocyte with cytoplasmic immunostaining (red arrow). C Immunostaining in the liver of PRV-1-infected specimens from farm 1 in CP2. PRV-1b antigens in hepatocytes (black arrow) and mononuclear-like cells (red arrow). Bar 50 μm. D Immunostaining in the spleen of PRV-1-infected specimens from farm 1 in CP3. PRV antigen detected with σ1 antibody in leukocyte-like cells (red arrow). Bar 50 μm.

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Veterinary Research

ISSN: 1297-9716