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Figure 6 | Veterinary Research

Figure 6

From: PRV-1b and PRV-3a infection is associated with the same clinical disease in coho salmon (Oncorhynchus kisutch) farmed in Chile: unraveling the pathogenesis of the orthoreoviral cardiomyopathy and hemolytic jaundice (OCHJ)

Figure 6

Spatial classification of different blood and plasma biomarkers of coho salmon specimens naturally challenged by PRV-1b and PRV-3a at different (temporal) checkpoints using principal coordinate analysis (PCA). The PCA did not show defined clusters when using the fish farm and positive/negative fish categories, so they are not shown. The PCAs were clustered into 4 groups: A The PCA shows that 60.9% of the total variance of the erythrogram was caught in two dimensions and the checkpoints contributed significantly to the total variance. The results of CP2 (yellow circles) formed a cluster independent of the results of CP1 (blue circles) and CP3 (green circles). The main biomarkers contributing to the total variation in dimension 1 were HGB, MCHC and viral load, while ECC and HTC contributed significantly to dimension 2. B The PCA shows that 68.8% of the total variance of the leukogram was caught in two dimensions. The checkpoints contributed significantly to the total variance, and CP2 (yellow circles) and CP3 (green circles) formed an independent cluster. Variables contributing significantly to the variance in dimension 1 were LCC, LYM, NEU and viral load, while MON contributed significantly to dimension 2. C PCA shows that 50.6% of the total variance of the serum enzyme profile was trapped in two dimensions and the checkpoints contributed significantly to the total variance. The results of CP2 (yellow circles) formed a cluster independent of the results of CP1 (blue circles) and CP3 (green circles). Serum enzymes contributing significantly to the total variation in dimension 1 were ALT, TCK and viral load, while LDH contributed significantly to dimension 2. D PCA shows that 62.9% of the total variance of the serum substrate profile was caught in two dimensions and that the checkpoints contributed significantly to the total variation. The results of CP2 (yellow circles) formed a cluster independent of the results of CP1 (blue circles) and CP3 (green circles). The serum substrates that contributed most significantly to the total variation in dimension 1 were TCH, HDL, LDL and TRG, whereas viral load, TPO, ALB, contributed significantly to dimension 2.

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Veterinary Research

ISSN: 1297-9716