Table 1 Comparative temporal-spatial frequency of PRV-1 and PRV-3 positivity rate (RT-PCR) and viral load (Ct) in heart and liver of seawater cultured coho salmon, clinical signs, macro- and microscopic pathological lesions, hematological and blood biochemical profile changes
Findings | PRV-1b | PRV-3a | ||||
|---|---|---|---|---|---|---|
Frequency (N° fish/Total; %) | Frequency (N° fish/N° Total, %) | |||||
Checkpoint 1 | Checkpoint 2 | Checkpoint 3 | Checkpoint 1 | Checkpoint 2 | Checkpoint 3 | |
Percentage of positive results (RT-PCR) and viral load expressed as cycle threshold (Ct; median) | ||||||
Heart | 13/24; 54,2% Ct 29,15 | 22/22; 100% Ct 18,40 | 20/21; 95,2% Ct 32,52 | 10/20; 50% Ct 28,26 | 10/20; 50% Ct 18,74 | 10/20; 90% Ct 32,11 |
Liver | 13/24; 54,2% Ct 28,01 | 22/22; 100% Ct 18,24 | 13/21; 61,9% Ct 32,75 | 11/20; 55% Ct 29,65 | 11/20; 55% Ct 19,16 | 11/20; 60% Ct 31,98 |
Heart + Liver | 14/24; 58,3% Ct 28,58 | 22/22; 100% Ct 18,32 | 13/21; 61,9% Ct 32,64 | 11/20; 55% Ct 28,95 | 11/20; 55% Ct 18,95 | 11/20; 75% Ct 32,05 |
Gross pathology | ||||||
Pale heart | 05/22; 22.7% | 09/21; 42.8% | 05/20; 25% | 09/20; 45% | ||
Heart rupture | 05/22; 22.7% | 11/21; 52.4% | 05/20; 25% | 11/20; 55% | ||
Hemopericardium | 06/22; 27.3% | 12/21; 57.1% | 05/20; 25% | 12/20; 60% | ||
Liver nutmeg | 04/22; 18.2% | 11/21; 52.4% | 04/20; 20% | 11/20; 55% | ||
Jaundice | 04/22; 18.2% | 11/21; 52.4% | 04/20; 20% | 11/20; 55% | ||
Ascites | 02/22; 9.1% | 08/21; 33.3% | 04/20; 20% | 08/20; 40% | ||
Clot in pericardial cavity | 03/22; 13.6% | 09/21; 42.8% | 03/20; 15% | 08/20; 40% | ||
Clot in abdominal cavity | 03/22; 13.6% | 08/21; 33.3% | 03/20; 15% | 08/20; 40% | ||
Biliary cholestasis | 02/22; 9.1% | 09/21; 42.8% | 02/20; 10% | 08/20; 40% | ||
Pale gills | 03/24; 12.5% | 06/22; 27.3% | 10/21; 47.6% | 02/20; 10% | 05/20; 25% | 09/20; 45% |
Histopathological findings | ||||||
Mononuclear hepatitis, low extension | 11/24; 45.8% | 11/22; 50% | 09/20; 45% | 11/20; 55% | ||
Mononuclear hepatitis, moderate-severe extension | 14/21; 66.7% | 12/20; 60% | ||||
Hepatic atrophy, low extension | 04/22; 18.2% | 03/20; 15% | ||||
Diffuse hepatic atrophy, moderate-severe extension | 19/21; 90.5% | 16/20; 80% | ||||
Mild infiltrate of melanomacrophagic cells in hepatic parenchyma, low extension | 11/22; 50% | 16/21; 76.2% | 10/20; 50% | 16/20; 80% | ||
Degeneration of cardiomyocytes in the ventricular spongy myocardium | 17/24; 70.8% | 12/20; 60% | ||||
Degeneration of cardiomyocytes in the ventricular spongy and compact myocardium, and in the atrium | 15/22; 68.2% | 16/21; 76.2% | 12/20; 60% | 16/20; 80% | ||
Mononuclear myocarditis, low extension | 04/24; 16.7% | 13/22; 59.1% | 03/20; 15% | 12/20; 60% | ||
Mononuclear myocarditis, moderate-severe extension | 17/21; 80.9% | 15/20; 85% | ||||
Diffuse mononuclear epicarditis, moderate | 12/21; 57.1% | 12/20; 60% | ||||
Endocardial hyperplasia, mild- moderate extension | 06/21; 28.6% | 07/20; 35% | ||||
Protein precipitate in tubular lumen and glomerular space | 16/24; 66.7% | 19/22; 86.4% | 11/20; 55% | 15/20; 85% | ||
Mesangial proliferative glomerylonephritis, low extension | 01/24; 4.2% | 04/22; 18.2% | 04/20; 20% | |||
Mild hyaline degeneration | 04/22; 18.2% | 04/20; 20% | ||||
Mild splenitis | 01/24; 4.2% | 11/22; 50% | 09/20; 45% | |||
Splenic congestion and multiple hemosiderin deposits, moderate extension | 20/21; 95.2% | 15/20; 85% | ||||
Mononuclear enteritis, low extension | 01/24; 4.2% | 01/22; 4.5% | 02/20; 10% | 02/20; 10% | ||
Chorionic edema in hindgut | 10/21; 47.5% | 10/20; 50% | ||||
Vacuolar depletion in hindgut enterocytes, low extesion | 01/22; 4.5% | 17/21; 80.9% | 02/20; 10% | 16/20; 80% | ||
Hemogram results | ||||||
Low hematocrit | 05/22; 22.7% | 06/21; 28.6% | 04/20; 20% | 04/20; 20% | ||
Low erythrocyte count | 05/22; 22.7% | 06/21; 28.6% | 04/20; 20% | 04/20; 20% | ||
Low hemoglobin concentration | 09/24; 37.5% | 12/22; 54.5% | 04/20; 20% | 08/20; 40% | ||
Low, mean corpuscular volume (MCV) | 10/24; 41.7% | 04/22; 18.2% | 04/21; 19.1% | 04/20; 20% | 05/20; 25% | 03/20; 15% |
Low, mean corpuscular hemoglobin concentration (MCHC) | 10/22; 45.5% | 05/20; 25% | ||||
Hypochromasia | 02/24; 8.3% | 03/20; 15% | ||||
Polychromasia | 03/21; 14.3% | 03/20; 15% | ||||
Poikilocytosis | 03/21; 14.3% | 03/20; 15% | ||||
Reactive lymphocytes | 05/24; 20.8% | 05/22; 22.7% | 05/21; 23.8% | 04/20; 20% | 05/20; 25% | 04/20; 20% |
Nuclear anomalies | 03/21; 14.3% | 03/20; 15% | ||||
Erythrocyte apoptosis | 06/24; 25% | 02/22; 9.1% | 03/21; 14.3% | 04/20; 20% | 10/20; 50% | 03/20; 15% |
Reticulocytes | 09/22; 40.1% | 10/21; 47.6% | 07/20; 35% | 08/20; 40% | ||
Anisocytosis | 04/21; 19.1% | 03/20; 15% | ||||
Leukopenia (low leukocytes count) | 05/22; 22.7% | 01/21; 4.8% | 02/20; 10% | 03/20; 15% | 01/20; 5% | |
Lymphopenia | 11/24; 45.8% | 05/21; 23.8% | 10/20; 50% | 05/20; 25% | ||
Lymphocytosis | 10/22; 45.5% | 10/20; 50% | ||||
Neutrophilia | 08/24; 25% | 06/20; 30% | ||||
Neutropenia | 10/22; 45.5% | 05/21; 23.8% | 09/20; 45% | 04/20; 20% | ||
Monocytosis | 07/22; 31.2% | 07/21; 33.3% | 05/20; 25% | 05/20; 25% | ||
Thrombocytopenia (low thrombocyte count) | 13/22; 59.1% | 10/20; 50% | ||||
Blood biochemical results | ||||||
Decreased LDL | 16/24; 66.7% | 20/22; 90.9% | 20/21; 95.2% | 16/20; 80% | 15/20; 85% | 18/20; 90% |
Increased ALP | 01/24; 4.2% | 03/22; 13.6% | 01/21; 4.8% | 02/20; 10% | 04/20; 20% | |
Increased LDH | 04/24; 16.7% | 03/22; 13.6% | 01/21; 4.8% | 02/20; 10% | 02/20; 10% | 01/20; 5% |
Increased ALT | 18/22; 81.8% | 03/21; 14.3% | 14/20; 70% | 02/20; 10% | ||
Decreased AST | 17/21; 80.9% | 14/20; 70% | ||||
Hypoproteinemia | 11/22; 50% | 07/20; 35% | ||||
Hypoalbuminemia | 13/22; 59.1% | 09/20; 45% | ||||
Total hyperbilirubinemia | 17/22; 77.3% | 16/21; 76.2% | 14/20; 70% | 14/20; 70% | ||