Figure 5

M. ovipneumoniae infection drives IFN-γ gene expression in blood and lungs. Gene expression analysis for a panel of immune related genes [34] was performed using quantitative RT-PCR, with the delta-delta Ct method for data analysis. A Whole blood samples were collected at weeks 0, 2, 4, 6 and 8 pi. Datapoints for each lamb represent average expression for weeks 2, 4, 6 and 8 pi normalized to the baseline samples collected at week zero before inoculation. B, C RNA was isolated from cranial and caudal lung tissues collected at necropsy (week 8 pi). Data were normalized to the average gene expression of the control group. For all three panels, individual data points, mean and SD are shown. Data were analyzed by 2-way ANOVA with Tukey’s multiple comparisons test (* P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001).