Figure 6

Identification of Mannheimia haemolytica in M. ovipneumoniae infected lambs. A Mannheimia haemolytica infection levels in nasal swab samples collected throughout the experiment were determined by qPCR. Data are shown as 40 minus Ct; individual data points, mean ± SD. Statistically significant differences between groups (* P ≤ 0.05) were determined by mixed-effects analysis with Tukey’s multiple comparisons test. Dotted line indicates limit of detection. B M. haemolytica infection levels in swab samples collected at necropsy from the nose, nasopharynx, trachea, and bronchi were determined by qPCR. Individual data points, mean ± SD. Statistically significant differences (** P ≤ 0.01) were determined by 2-way ANOVA with Tukey’s multiple comparisons test. C Timepoint for first detection of a PCR-positive sample (Ct ≤ 35) for M. ovipneumoniae or M. haemolytica in each animal. Only lambs from the URT and LRT groups were included in the analysis. Individual datapoints, mean ± SD; * P ≤ 0.05 Wilcoxon matched-pairs signed rank test.