Figure 14

AUROC curves predicting CNS disorders based on CSF Nf-L concentration. The AUROC for CSF Nf-L concentration in calves with a CNS anomaly and a neurodegenerative disorder was 1 (light blue line) and the corresponding optimal cut-off to distinguish between them was 3495 pg/mL (Se, 100%, Sp, 100%). The AUROC for CSF Nf-L concentration in calves with a CNS anomaly and with a CNS infection was 0.83 (green line) and the corresponding optimal cut-off to distinguish between them was 467.5 pg/mL (Se, 84.21%, Sp, 61.54%. The AUROC for CSF Nf-L concentration in calves aged 2–12 months with a metabolic/toxic disorder and a CNS infection was 0.87 (red line) and the corresponding optimal cut-off to distinguish between them was 966.5 pg/mL (Se, 68.18%, Sp, 84.62%). The x-axis represents the false positive rate (1-Specificity), and the y-axis represents the true positive rate (Sensitivity). ANOM denotes anomalies, AUROC area under the receiver operating characteristic curve, CSF cerebrospinal fluid, CNS central nervous system, DEG degenerative, INF/INFLAMM infectious/inflammatory, MET/TOX metabolic/toxic, Nf-L neurofilament light chain, Se sensitivity, Sp specificity.