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Figure 2 | Veterinary Research

Figure 2

From: Deltacoronavirus HKU11, HKU13, PDCoV (HKU15) and HKU17 spike pseudoviruses enter avian DF-1 cells via clathrin-mediated endocytosis in a Rab5-, Rab7- and pH-dependent manner

Figure 2

CME pathways involved in HKU11, HKU13 and HKU17 pseudovirus entry into DF-1 cells. DF-1 cells were pretreated with the indicated concentrations of A CPZ (CME inhibitor), B latrunculin B (macropinocytosis inhibitor), or C nystatin (CavME inhibitor) for 1 h and infected with retroviruses pseudotyped with selected DCoV spike proteins. The internalization of VSV-G pseudovirions via CME was used as a control. D siCHC- or siCtrl-transfected cells were infected with selected pseudoviruses. At 36 hpi, the cells were lysed to determine luciferase activity. E The expressed CHC or HIV p24 proteins were analysed by western blot; error bars indicate SEM (two-tailed t test, *p < 0.05, ***p < 0.001; n ≥ 3).

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Veterinary Research

ISSN: 1297-9716