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Figure 3 | Veterinary Research

Figure 3

From: Deltacoronavirus HKU11, HKU13, PDCoV (HKU15) and HKU17 spike pseudoviruses enter avian DF-1 cells via clathrin-mediated endocytosis in a Rab5-, Rab7- and pH-dependent manner

Figure 3

HKU11, HKU13 and HKU17 pseudovirus entry depends on dynamin-2. A DF-1 cells were pretreated with dynasore for 1 h at the indicated concentrations before pseudovirus infection, and the amount of virus endocytosed was measured by measuring luciferase activity at 48 hpi. B DF-1 cells were transfected with Flag-tagged WT dynamin-2 (DynII-WT) or DN mutant dynamin-2 (DynII-DN). At 24 h post-transfection, the cells were infected with selected pseudoviruses. The cell entry efficiency was measured by measuring the luciferase activity after 48 h. C The expression of these proteins as well as p24 was analysed via western blotting with an anti-Flag antibody; error bars indicate the SEM (two-tailed t test, *p < 0.05, ***p < 0.001; n ≥ 3).

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Veterinary Research

ISSN: 1297-9716