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Figure 5 | Veterinary Research

Figure 5

From: Deltacoronavirus HKU11, HKU13, PDCoV (HKU15) and HKU17 spike pseudoviruses enter avian DF-1 cells via clathrin-mediated endocytosis in a Rab5-, Rab7- and pH-dependent manner

Figure 5

Rab5 and Rab7 are necessary for HKU11, HKU13 and HKU17 pseudovirus infection. A, B Cells transfected with plasmids expressing Flag-tagged Rab5 and Rab7 (WT or DN constructs) were then infected with select avian DCoV pseudoviruses. C, D The expression of these proteins as well as p24 was analysed via western blotting with anti-Flag antibodies. E siRab5- or F siRab7-transfected cells were infected with selected pseudoviruses, and the cell entry efficiency was determined by luciferase activity measurement after 36 hpi. G, H The knockdown efficiency of the siRNAs was measured by western blotting; error bars indicate SEM (two-tailed t test, *p < 0.05, ***p < 0.001; n ≥ 3).

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Veterinary Research

ISSN: 1297-9716