Table 1 Construction of mutant plasmids
Primer | Sequence (5’–3’) |
|---|---|
g-DNMII-arm-F | tccactagtccagtgtggtggaattcgccaccatggggaaccgcggcatggaggagctgatcccgctggt |
g-DNMII-arm-R | tcagcgggtttaaacgggccctctagattacttatcgtcgtcatccttgtaatcgtcgagcagggagggct |
g-DNMII-mut-F | tgccgcagatcgccgtggtgggcgggcagagcgcggggaagagctccgtgctggagaacttcgt |
g-DNMII-mut-R | gaagttctccagcacggagctcttccccgcgctctgcccgcccaccacggcgatctgcggcaggt |
g-Rab5-WT-F | atggctaatcgtggagcaacaagacccaacgggccaaat |
g-Rab5-WT-R | cttatcgtcgtcatccttgtaatcgttactacaacatt |
g-Rab7-WT-F | atgacttctaggaagaaagtgttact |
g-Rab7-WT-R | cttatcgtcgtcatccttgtaatcgcagctgcagctct |
g-Rab5-DN-F | agtctgcagttggtaaaaacagtttggtgct |
g-Rab5-DN-R | agcaccaaactgtttttaccaactgcagact |
g-Rab7-DN-F | gtggggaagaactcactcatgaaccagt |
g-Rab7-DN-R | tcatgagtgagttcttccccaccccagagt |
g-Rab5-CA-F | atacagctgggctagagcggtat |
g-Rab5-CA-R | ataccgctctagcccagctgtat |
g-Rab7-CA-F | atacagcaggcctagaacgattccagtct |
g-Rab7-CA-R | agactggaatcgttctaggcctgctgtat |