Skip to main content
Figure 4 | Veterinary Research

Figure 4

From: TRIM8 inhibits porcine epidemic diarrhoea virus replication by targeting and ubiquitinately degrading the nucleocapsid protein

Figure 4

TRIM8 catalyses PEDV N protein degradation via K48-linked ubiquitination. A Co-IP and IB analysis of HEK293T cells expressing HA-tagged PEDV N protein and increasing amounts of TRIM8. B IB analysis of cells expressing HA-tagged PEDVN with or without TRIM8 under CHX treatment for different time points (0, 9, 12, 15 h). CHX: Cycloheximide. C IB analysis of cells expressing HA-tagged PEDV N under the treatment of proteasome inhibitor MG132 for 12 h. D IB analysis of cells expressing HA-tagged PEDV N under the treatment of autophagy-lysosome inhibitor Leupeptin for 12 h. E Co-IP and IB analysis of MG132-treated cells expressing MYC-tagged PEDV N, with or without TRIM8, HA-tagged wild-type ubiquitin, mutant ubiquitin K48 and K63. WCL: whole cell lysates.

Back to article page

Veterinary Research

ISSN: 1297-9716