Figure 5
Effects of rKaSPI and rAdSPI on TJ and Mucin expression in IPECs. rKaSPI and rAdSPI were incubated with IPECs for 24 h, respectively, before carrying out the assays. A RT-qPCR, B Western blotting, and C Immunofluorescence (magnification: 4 × PL FL; scale bar: 1000 μm) analysed the expression of TJs in IPECs treated with rKaSPI and rAdSPI. D RT-qPCR, E Western blotting and F Immunofluorescence (magnification: 4 × PL FL; scale bar: 1000 μm) analysed the expression of Mucins in IPECs treated with rKaSPI and rAdSPI. Assays were performed in triplicate, and the data were expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the IPECs + PBS group; &P < 0.05, &&P < 0.01, &&&P < 0.001 compared with the IPECs + rKaSPI group.