Table 2 Primers of the detected genes in IPEC-J2 cells.
Gene name | Primers | Sequence | Access number |
|---|---|---|---|
GAPDH | Forward | 5ʹ-GGTGAAGGTCGGAGTGAACG-3ʹ | NM_001206359.1 |
Reverse | 5ʹ-CCGTGGGTGGAATCATACTGG-3ʹ | ||
IL-1β | Forward | 5ʹ-AAAGATAACACGCCCACCC-3ʹ | NM_214055.1 |
Reverse | 5ʹ-GGAGTTTCCCAGGAAGACG-3ʹ | ||
TNF-α | Forward | 5ʹ-GCTCTTCTGCCTACTGCACTTC-3ʹ | NM_214022.1 |
Reverse | 5ʹ-GCTGTCCCTCGGCTTTGA-3ʹ | ||
TGF-β | Forward | 5ʹ-ATTTACTTACTGAGCATCTTGGACCTTA-3ʹ | NM_214015.2 |
Reverse | 5ʹ-GGGTGTTATCAGAGTCCCTTTTAGC-3ʹ | ||
IL-10 | Forward | 5ʹ-GACTCAACGAAGAAGGCACAG-3ʹ | NM_214041.1 |
Reverse | 5ʹ-GCAGGCTGGTTGGGAAGT-3ʹ | ||
ZO-1 | Forward | 5ʹ-AGGTGCTCCCATCGT-3ʹ | XM_021098827 |
Reverse | 5ʹ-TTTCGGTAATACTCTTCATC-3ʹ | ||
ZO-2 | Forward | 5ʹ-GGAGCATTGACCCGACTTAC-3ʹ | NM_001206404 |
Reverse | 5ʹ-AGACCATACTCTTCATTCGCTTT-3ʹ | ||
claudin-3 | Forward | 5ʹ-CAGAGCCGTTCGCAACCAGG-3ʹ | NM_001160075.1 |
Reverse | 5ʹ-CACCACGCAGTTCATCCACAGG-3ʹ | ||
occludin | Forward | 5ʹ-CACCCAGCAACGACA-3ʹ | NM_001163647 |
Reverse | 5ʹ-ATAACGAGCATAGACAGAAT-3ʹ | ||
TLR1 | Forward | 5ʹ-CTCTGCTCAAGGACTTCCGTGTA-3ʹ | NM_001031775.1 |
Reverse | 5ʹ-AGAGCCAGTGCCAGCCCAGT-3ʹ | ||
TLR2 | Forward | 5ʹ-GGTCCGATGCTGGTCTTTATC-3ʹ | NM_213761.1 |
Reverse | 5ʹ-GCAAGTCACCCTTTATGTTATTCA-3ʹ | ||
TLR4 | Forward | 5ʹ-CCAGTGCTGCTTTGAATAGAG-3ʹ | NM_001293316.1 |
Reverse | 5ʹ-GAACAGAAGTGACCCGGAGA-3ʹ | ||
MUC-1 | Forward | 5ʹ-ATGAGCTGGGAGCACAGGTGG-3ʹ | XM_001926883.5 |
Reverse | 5ʹ-CCAGGCTCGGATGGACTTCG-3ʹ | ||
MUC-2 | Forward | 5ʹ-AGGACGACACCATCTACCTCACTCA-3ʹ | XM_013989745.1 |
Reverse | 5ʹ-GCAAGGCCAGCTCGGGAAT-3ʹ |