Figure 1

Binding of u-PA and its precursor by FhNEJ-Teg proteins. A, B Binding of FhNEJ-Teg proteins to pro-u-PA (A) or u-PA (B) was detected via ELISA by coating wells with FhNEJ-Teg (0.5 µg) and incubating with increasing amounts of these factors. In parallel, a condition where ε-ACA was present during pro-u-PA/u-PA incubation was included to assess whether the binding of these fibrinolytic factors to FhNEJ-Teg proteins occurs via lysine residues. Wells coated with 1% BSA served as controls for nonspecific binding to pro-u-PA/u-PA. The graphs are representative of two independent experiments. The data points indicate the means of three technical replicates ± SD, purple asterisks indicate significant differences between pro-u-PA/u-PA and the negative control (1% BSA), and black asterisks indicate significant differences between the negative control (1% BSA) and pro-u-PA/u-PA incubated in the presence of ε-ACA (**p ≤ 0.1; ***p ≤ 0.001; one-way ANOVA). Note that, in Panel B, the data points corresponding to FhNEJ-Teg and FhNEJ-Teg + ε-ACA overlap.